Methods
SeqTRACS is implemented in the Java programming language as a fat client with an Oracle RDMS backend. The tracefile viewer uses modified classes from the BioJava open source project. (http://www.biojava.org/). In order to perform the base calling and calculate quality scores, seqtracs relies on the commonly used phred software to analyze the tracefiles (Ewing et al. 1998). The SeqTRACS GUI uses Java Webstart for easy deployment and updating while avoiding the restrictions associated with Java applets. (http://java.sun.com/products/javawebstart/index.jsp).